Practical method for cloning cDNAs generated in an mRNA differential display.

assembly with dimensions of 14 x 17 in. can accommodate 12 high-density arrayed filters (8 x 12 cm) or 15 circular filters (82 mm diameter). The overall results produce a significant increase in the throughput of the number of filters that can be processed with consistent results and the absence of any cross contamination. Cleaning the netting assembly is easily accomplished by rinsing with water and air drying. Compressed air can be used for drying as an alternative to air drying. We have successfully used this device with consistent results for the analysis of high-density arrayed filters or colony lifts using filter circles (Figure 2). The vast amount of information being generated as a result of the advances in the human genome project necessitates rapid methods to facilitate the analysis of a large number of clones. This device can be easily adapted to an automated workstation designed to carry out the processing of a large number of filters. In summary, this simple and inexpensive device should be a valuable tool to aid in the rapid analysis of specific clones in the number of laboratories engaged in genome research.